Pukke surged to the top of the Varsity Cricket table after retaining their unbeaten run in all three of their matches in Potchefstroom on Wednesday.
They also claimed a bonus point in two of their games.
Tuks are second, also unbeaten, but with fewer points while Maties sitting pretty at third with two wins out of four in their matches played.
UJ are fourth with two wins out of three games and eight points while NMMU and UCT are tied on four points, but NMMU ahead on net run rate.
Two teams are winless in the tournament thus far, with UWC still without a single point after two matches and Kovsies also without points after three games.
The action continues on Thursday with six matches to be played.
Maties vs UWC: Maties won by 45 runs
Maties: 181/6 (I Gafieldien 37*, L Le Roux 31; I Hendricks 2/21, L Williams 1/22)
UWC: 136/7 (J Smith 31, I Hendricks 21; N Botha 4/22, E Schutte 2/21)
Pukke vs Kovsies: Pukke won by 60 runs
NWU Pukke: 188/9 (R Kruger 50, W Lubbe 39; J van Dyk 3/45, K Rafferty 2/26)
UFS Kovsies: 127 all out (C Dry 29, A Muller 20; Du Plooy 2/12, Fortuin 2/31)
Tuks vs UJ: Tuks won by 8 runs
UP-Tuks: 150/6 (A Markram 53, J Wessels 26; I Moseki 3/28, M Varner 2/31)
UJ: 142/8 (U Asad 37*, J Broodryk 37; C Bosch 3/22, L Ngidi 1/13)
UCT vs NMMU: NMMU won by six wicket (with two balls remaining)
UCT: 137/9 (S Golding 33, L Stevens 30; E Moore 3/27, J Davidson 2/26)
NMMU: 140/4 (M Christensen 50, E Moore 28; L Stevens 2/24, N Madwe 1/23)
Maties vs Kovsies: Maties won by 120 runs
Maties: 237-2 (J Marais 107*, D Bedingham 61; M Bruwer 1/36, C Dry 1/39)
Kovsies: 117 all out (D Bruwer 51, M Bruwer; E Schutte 3/12, N Botha 3/33)
Pukke vs UCT: Pukke won by 40 runs
Pukke: 119/5 (H du Plooy 37, R Kruger 26; L Stevens 1/19, S Golding 1/23)
UCT: 84 all out (J Strang 14, J Leppan 14; B Fortuin 2/9, J van Wyngaardt 4/16)